Composite

Part:BBa_K2557021:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


Ubc promoter-Bxb1-RDF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1974
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1974
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1182
    Illegal BglII site found at 1980
    Illegal BamHI site found at 408
    Illegal BamHI site found at 623
    Illegal BamHI site found at 939
    Illegal BamHI site found at 1647
    Illegal BamHI site found at 1938
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1974
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1974
    Illegal NgoMIV site found at 924
    Illegal NgoMIV site found at 2457
    Illegal NgoMIV site found at 2640
    Illegal AgeI site found at 414
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

In order to allow the recombinase to enter the nucleus and function, we added SV40 NLS to the front end. To detect protein content, we added the FLAG tag.


Source

The Ubc promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the sequence of Bxb1-RDF from the literature and synthesized this sequence.

References